Polaromonas sp. strain JS666 is the only organism thus-far known that can couple growth to aerobic oxidation of cis-dichlorothene (cDCE). As such, it is an attractive candidate as a bioaugmentation agent at sites where reductive dechlorination has stalled at cDCE and it has migrated to aerobic zones. This presentation reports the successful use of JS666 as a bioaugmentation agent for stimulating cDCE-oxidation in soil microcosms - small-scale surrogates of larger-scale, subsurface systems - constructed with materials from five cDCE-contaminated sites. Survival and growth of JS666 were tracked with quantitative, real-time PCR (based on the isocitrate lyase gene of JS666). The presentation also includes ongoing studies directed at elucidation of the cDCE-degradation pathway(s) in JS666.